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PNPase antibody

Anti-PNPase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118737
Anti-PNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX118737
Anti-PNPase antibody used in Immunoprecipitation (IP). GTX118737
Anti-PNPase antibody used in Western Blot (WB). GTX118737

Cat. No. GTX118737

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PNPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PNPase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617865

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polyribonucleotide nucleotidyltransferase 1 , COXPD13 , DFNB70 , OLD35 , PNPASE , old-35

Cellular Localization

Mitochondrion

Background

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PNPase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118737

GTX118737 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase(GTX118737) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-PNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX118737

GTX118737 IHC-P Image

PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis.
Sample: Paraffin-embedded human normal colon.
PNPase antibody (GTX118737) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PNPase antibody used in Immunoprecipitation (IP). GTX118737

GTX118737 IP Image

Immunoprecipitation of PNPase protein from HeLa whole cell extracts using 5 μg of PNPase antibody (GTX118737).
Western blot analysis was performed using PNPase antibody (GTX118737).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PNPase antibody used in Western Blot (WB). GTX118737

GTX118737 WB Image

PNPase antibody detects PNPase protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PNPase antibody (GTX118737) diluted by 1:2500.

REFERENCE

Application Reference

D Giosa et al. Commun Biol 2023; 6 (1) : 684 Mitochondrial DNA is a target of HBV integration
Application : WB
Wang DD et al. J Biol Chem 2014; Helicase SUV3, Polynucleotide Phosphorylase PNPase, and Mitochondrial Polyadenylation Polymerase mtPAP Form a Transient Complex to Modulate Mitochondrial mRNA Polyadenylated Tail Lengths in Response to Energetic Changes.
Application : IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169