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PODXL antibody [2A4]

Anti-PODXL antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX34979
Anti-PODXL antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX34979

Cat No. GTX34979

Host Mouse
Clonality Monoclonal
Clone Name 2A4
Isotype IgM
Application ICC/IF, IHC-P
Reactivity Human, Rat, Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Specificity/Sensitivity

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Purification

Ab purified from Bioreactor Concentrate

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Podocalyxin Like,Gp200,Pc,Pclp,Pclp-1,Podxl

Background

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PODXL antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX34979

GTX34979 IHC-P Image

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4).

Anti-PODXL antibody [2A4] used in IHC (Paraffin sections) (IHC-P). GTX34979

GTX34979 IHC-P Image

Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Monoclonal Antibody (2A4).

Package List Price ($)
$ 329