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PODXL antibody [4F10]

Anti-PODXL antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX34978

Cat. No. GTX34978

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F10

Isotype

 IgM

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Rabbit
Package
100 μg ($529)
See all PODXL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Purification

Purified IgM

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Gp200 , PC , PCLP , PCLP1 , PODXL , podocalyxin like , Podocalyxin , GCTM-2 antigen , GCTM2

Cellular Localization

Apical cell membrane,Cell projection,Lamellipodium,Filopodium,Ruffle,Microvillus,Membrane raft,Membrane

Background

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PODXL antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX34978

GTX34978 IHC-P Image

IHC-P analysis of human angiosarcoma tissue using GTX34978 PODXL antibody [4F10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529