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PODXL antibody [C2C3], C-term

Anti-PODXL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104764
Anti-PODXL antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104764
Anti-PODXL antibody [C2C3], C-term used in Western Blot (WB). GTX104764

Cat No. GTX104764

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:500
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

human ESC

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PODXL. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

podocalyxin like , Gp200 , PC , PCLP , PCLP-1

Background

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PODXL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104764

GTX104764 IHC-P Image

PODXL antibody [C2C3], C-term detects PODXL protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
PODXL antibody [C2C3], C-term (GTX104764) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PODXL antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104764

GTX104764 ICC/IF Image

Immunofluorescence analysis of methanol-fixed mouse ESC, using PODXL(GTX104764) antibody at 1:100 dilution.

Anti-PODXL antibody [C2C3], C-term used in Western Blot (WB). GTX104764

GTX104764 WB Image

Sample (20 µg of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX104764 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
There are currently no references for PODXL antibody [C2C3], C-term (GTX104764). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PODXL antibody [C2C3], C-term (GTX104764). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169