APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human PODXL Intracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human PODXL. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
podocalyxin like , Gp200 , PC , PCLP , PCLP-1
Cellular Localization
Apical cell membrane,Cell projection, lamellipodium,Cell projection, filopodium,Membrane
Background
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951](/templates/default/js/lightbox/images/loading.gif "Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951")
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GTX637951 WB Image
Wild-type (WT) and PODXL knockout (KO) HeLa cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PODXL antibody [HL2047] (GTX637951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637951 IHC-P Image
PODXL antibody [HL2047] detects PODXL protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.PODXL stained by PODXL antibody [HL2047] (GTX637951) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637951 IHC-P Image
PODXL antibody [HL2047] detects PODXL protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.PODXL stained by PODXL antibody [HL2047] (GTX637951) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637951 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PODXL antibody [HL2047] (GTX637951) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637951 IHC-P Image
PODXL antibody [HL2047] detects PODXL protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.PODXL stained by PODXL antibody [HL2047] (GTX637951) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for PODXL antibody [HL2047] (GTX637951). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PODXL antibody [HL2047] (GTX637951). Be the first to share your experience with this product.
![Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951](/upload/website/prouct_img/small/GTX637951/GTX637951_T-44886_20230106_WB_KO_watermark_23010922_258.webp "Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951")
![Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951](/upload/website/prouct_img/small/GTX637951/GTX637951_44963_20230714_IHC-P_Cat_23081619_566.webp "Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951")
![Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951](/upload/website/prouct_img/small/GTX637951/GTX637951_T-44886_20221230_IHC-P_M_23020621_467.webp "Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951")
![Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951](/upload/website/prouct_img/small/GTX637951/GTX637951_44963_20230317_WB_TPM_watermark_23032022_476.webp "Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951")
![Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951](/upload/website/prouct_img/small/GTX637951/GTX637951_44963_20230419_IHC-P_R_23053001_691.webp "Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951")
![Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951](/upload/website/prouct_img/normal/GTX637951/GTX637951_T-44886_20230106_WB_KO_watermark_23010922_258.webp "Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951")
![Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951](/upload/website/prouct_img/normal/GTX637951/GTX637951_44963_20230714_IHC-P_Cat_23081619_566.webp "Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951")
![Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951](/upload/website/prouct_img/normal/GTX637951/GTX637951_T-44886_20221230_IHC-P_M_23020621_467.webp "Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951")
![Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951](/upload/website/prouct_img/normal/GTX637951/GTX637951_44963_20230317_WB_TPM_watermark_23032022_476.webp "Anti-PODXL antibody [HL2047] used in Western Blot (WB). GTX637951")
![Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951](/upload/website/prouct_img/normal/GTX637951/GTX637951_44963_20230419_IHC-P_R_23053001_691.webp "Anti-PODXL antibody [HL2047] used in IHC (Paraffin sections) (IHC-P). GTX637951")