GeneTex
United States (US)

POFUT1 antibody

Anti-POFUT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31776
Anti-POFUT1 antibody used in Western Blot (WB). GTX31776

Cat No. GTX31776

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide

Specificity/Sensitivity

At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

POFUT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1.The immunogen is located within amino acids 90 - 140 of POFUT1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein O-Fucosyltransferase 1,Ddd2,Fut12,O-Fut,O-Fuc-T,O-Fuct-1,Ofuct1,Pofut1

Background

This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-POFUT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31776

GTX31776 IHC-P Image

IHC-P analysis of human heart tissue using GTX31776 POFUT1 antibody.
Working concentration : 2.5 μg/ml

Anti-POFUT1 antibody used in Western Blot (WB). GTX31776

GTX31776 WB Image

WB analysis of human heart tissue lysate using GTX31776 POFUT1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Package List Price ($)
$ 329