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POFUT2 antibody

Anti-POFUT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64979
Anti-POFUT2 antibody used in Western Blot (WB). GTX64979
Anti-POFUT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64979

Cat. No. GTX64979

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all POFUT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein O-fucosyltransferase 2 , C21orf80 , FUT13

Cellular Localization

Endoplasmic reticulum,Golgi apparatus

Background

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-POFUT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64979

GTX64979 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX64979 POFUT2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-POFUT2 antibody used in Western Blot (WB). GTX64979

GTX64979 WB Image

WB analysis of various sample lysates using GTX64979 POFUT2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POFUT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64979

GTX64979 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64979 POFUT2 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399