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POLD3 antibody

Anti-POLD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32795
Anti-POLD3 antibody used in Western Blot (WB). GTX32795
Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795
Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795

Cat. No. GTX32795

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all POLD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase delta 3, accessory subunit , P66 , P68 , PPP1R128

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-POLD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32795

GTX32795 IHC-P Image

IHC-P analysis of human oophoroma tissue using GTX32795 POLD3 antibody.
Dilution : 1:100

Anti-POLD3 antibody used in Western Blot (WB). GTX32795

GTX32795 WB Image

WB analysis of various sample lysates using GTX32795 POLD3 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795

GTX32795 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32795 POLD3 antibody.

Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795

GTX32795 ICC/IF Image

ICC/IF analysis of GFP-RNF168 transgenic U2OS cells using GTX32795 POLD3 antibody.
Green : GFP-RNF168 fusion protein expression for DNA damage marker
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399