GeneTex
United States (US)

POLD3 antibody

Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795
Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795
Anti-POLD3 antibody used in Western Blot (WB). GTX32795
Anti-POLD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32795

Cat No. GTX32795

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

51 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Polymerase Delta 3, Accessory Subunit,P66,P68,Ppp1R128,Pold3

Background

This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

DATA IMAGES
Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795

GTX32795 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32795 POLD3 antibody.

Anti-POLD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32795

GTX32795 ICC/IF Image

ICC/IF analysis of U2OS cell expressing GFP tagged RNF168 protein using GTX32795 POLD3 antibody.RNF168 (GFP) can be used to mark cells damage by UV-A treatment.
Green : RNF168
Blue : DAPI

Anti-POLD3 antibody used in Western Blot (WB). GTX32795

GTX32795 WB Image

WB analysis of various samples using GTX32795 POLD3 antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POLD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32795

GTX32795 IHC-P Image

IHC-P analysis of human oophoroma tissue using GTX32795 POLD3 antibody.
Dilution : 1:100

Package List Price ($)
$ 329