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POLE antibody [3C5.1]

Anti-POLE antibody [3C5.1] used in Western Blot (WB). GTX30112

Cat. No. GTX30112

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3C5.1

Isotype

 IgG2a

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Mustelid, Primate
Package
50 μl ($419)
See all POLE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 5 μg/ml
ICC/IF 1:100
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

262 kDa. ( Note )

Product Note

This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
We do not recommend use of this product for Drosophila samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human DNA Polymerase epsilon purified from HeLa cells.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase epsilon, catalytic subunit , CRCS12 , FILS , IMAGEI , POLE1

Cellular Localization

Nucleus

Background

This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-POLE antibody [3C5.1] used in Western Blot (WB). GTX30112

GTX30112 WB Image

WB analysis of HeLa cell lysate using GTX30112 POLE antibody [3C5.1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 419
Custom Conjugations