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POLE antibody [HL2150]

Recombinant antibody Orthogonal
Anti-POLE antibody [HL2150] used in Western Blot (WB). GTX638128
Anti-POLE antibody [HL2150] used in Western Blot (WB). GTX638128
Anti-POLE antibody [HL2150] used in IHC (Paraffin sections) (IHC-P). GTX638128

Cat. No. GTX638128

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2150

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all POLE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

262 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human POLE.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase epsilon, catalytic subunit , CRCS12 , FILS , IMAGEI , POLE1

Cellular Localization

Nucleus

Background

This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-POLE antibody [HL2150] used in Western Blot (WB). GTX638128

GTX638128 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody [HL2150] (GTX638128) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-POLE antibody [HL2150] used in Western Blot (WB). GTX638128

GTX638128 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody [HL2150] (GTX638128) diluted at 1:900. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-POLE antibody [HL2150] used in IHC (Paraffin sections) (IHC-P). GTX638128

GTX638128 IHC-P Image

POLE antibody [HL2150] detects POLE protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
POLE stained by POLE antibody [HL2150] (GTX638128) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169