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POLE antibody

Anti-POLE antibody used in Western Blot (WB). GTX132100
Anti-POLE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132100
Anti-POLE antibody used in IHC (Paraffin sections) (IHC-P). GTX132100

Cat No. GTX132100

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

262 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POLE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DNA polymerase epsilon, catalytic subunit,CRCS12,FILS,IMAGEI,POLE1

Cellular Localization

Nucleus

Background

Participates in DNA repair and in chromosomal DNA replication.

Database

Research Area

DATA IMAGES
Anti-POLE antibody used in Western Blot (WB). GTX132100

GTX132100 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody (GTX132100) diluted at 1:1000.

Anti-POLE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132100

GTX132100 ICC/IF Image

POLE antibody detects POLE protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: POLE protein stained by POLE antibody (GTX132100) diluted at 1:2500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-POLE antibody used in IHC (Paraffin sections) (IHC-P). GTX132100

GTX132100 IHC-P Image

POLE antibody detects POLE protein at nucleus in human ovary by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
POLE antibody (GTX132100) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169