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POLH antibody [5H10]

Anti-POLH antibody [5H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00871
Anti-POLH antibody [5H10] used in Western Blot (WB). GTX00871

Cat. No. GTX00871

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H10

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all POLH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:200-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant full-size Pol eta tagged with His6

Purification

Purified by salting-out and ion-exchange chromatography
From culture supernant

Purity

90-95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase eta , POLH , RAD30 , RAD30A , XPV

Cellular Localization

Nucleus

Background

This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-POLH antibody [5H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00871

GTX00871 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00871 POLH antibody [5H10].
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS, 10 min
Dilution : 1:1000

Anti-POLH antibody [5H10] used in Western Blot (WB). GTX00871

GTX00871 WB Image

WB analysis of various samples using GTX00871 POLH antibody [5H10].
Lane 1 : Recombinant full-size Pol eta (POLH) with 6XHis tag at C-teminus
Lnae 2 : HeLa whole cell lysate (~40 μg)
Lane 3 : XP2SA (XP-V) whole cell lysate (~40 μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399