WB analysis of HeLa whole cell lysate using GTX48495 POLK antibody.
Loading : 35 μg
Dilution : 1:1000

Western blot using GeneTex's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 μg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4º followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.