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POLR1D antibody [N1C3]

Anti-POLR1D antibody [N1C3] used in Western Blot (WB). GTX115993
Anti-POLR1D antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115993

Cat. No. GTX115993

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all POLR1D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human POLR1D (AAH00889) Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620488

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase I and III subunit D , AC19 , POLR1C , RPA16 , RPA9 , RPAC2 , RPC16 , RPO1-3 , TCS2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-POLR1D antibody [N1C3] used in Western Blot (WB). GTX115993

GTX115993 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
15% SDS PAGE
GTX115993 diluted at 1:1000

Anti-POLR1D antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115993

GTX115993 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POLR1D(GTX115993) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169