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POLR2C antibody [N1C3-2]

Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715
Anti-POLR2C antibody [N1C3-2] used in Western Blot (WB). GTX118715
Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715
Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715
Anti-POLR2C antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118715
Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715
Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715

Cat. No. GTX118715

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all POLR2C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human POLR2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176211

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit C , RPB3 , RPB31 , hRPB33 , hsRPB3

Cellular Localization

Nucleus

Background

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715

GTX118715 IHC-P Image

POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
POLR2C antibody [N1C3-2] (GTX118715) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POLR2C antibody [N1C3-2] used in Western Blot (WB). GTX118715

GTX118715 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX118715 diluted at 1:1000

Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715

GTX118715 IHC-P Image

POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
POLR2C antibody [N1C3-2] (GTX118715) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715

GTX118715 IHC-P Image

POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
POLR2C antibody [N1C3-2] (GTX118715) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POLR2C antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118715

GTX118715 ICC/IF Image

POLR2C antibody [N1C3-2] detects POLR2C protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: POLR2C protein stained by POLR2C antibody [N1C3-2] (GTX118715) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715

GTX118715 IHC-P Image

POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
POLR2C antibody [N1C3-2] (GTX118715) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POLR2C antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118715

GTX118715 IHC-P Image

POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
POLR2C antibody [N1C3-2] (GTX118715) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169