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POLR2E antibody

Anti-POLR2E antibody used in Western Blot (WB). GTX54591
Anti-POLR2E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54591
Anti-POLR2E antibody used in IHC (Paraffin sections) (IHC-P). GTX54591

Cat. No. GTX54591

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all POLR2E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit E , RPABC1 , RPB5 , XAP4 , hRPB25 , hsRPB5

Cellular Localization

Nucleus

Background

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Database

DATA IMAGES

Anti-POLR2E antibody used in Western Blot (WB). GTX54591

GTX54591 WB Image

WB analysis of various sample lysates using GTX54591 POLR2E antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POLR2E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54591

GTX54591 ICC/IF Image

ICC/IF analysis of A549 cells using GTX54591 POLR2E antibody.

Anti-POLR2E antibody used in IHC (Paraffin sections) (IHC-P). GTX54591

GTX54591 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX54591 POLR2E antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399