GeneTex
United States (US)

POLR2E antibody

GTX54591_20180530_IHC-P_18121411_671.jpg
GTX54591_20180530_ICCIF_18121411_322.jpg
GTX54591_20180530_WB_18121411_688.jpg

Cat No. GTX54591

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rna Polymerase Ii Subunit E,Rpabc1,Rpb5,Xap4,Hrpb25,Hsrpb5,Polr2E

Background

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX54591_20180530_IHC-P_18121411_671.jpg

GTX54591 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX54591 POLR2E antibody.
Dilution : 1:100

GTX54591_20180530_ICCIF_18121411_322.jpg

GTX54591 ICC/IF Image

ICC/IF analysis of A549 cell using GTX54591 POLR2E antibody.

GTX54591_20180530_WB_18121411_688.jpg

GTX54591 WB Image

WB analysis of various samples using GTX54591 POLR2E antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 319