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POLR2G antibody

Anti-POLR2G antibody used in IHC (Paraffin sections) (IHC-P). GTX108874
Anti-POLR2G antibody used in IHC (Paraffin sections) (IHC-P). GTX108874
Anti-POLR2G antibody used in Western Blot (WB). GTX108874

Cat. No. GTX108874

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all POLR2G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Zebrafish, Sheep, Drosophila, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human POLR2G Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951315

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit G , RPB19 , RPB7 , hRPB19 , hsRPB7

Cellular Localization

Nucleus

Background

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-POLR2G antibody used in IHC (Paraffin sections) (IHC-P). GTX108874

GTX108874 IHC-P Image

POLR2G antibody detects POLR2G protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
POLR2G antibody (GTX108874) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-POLR2G antibody used in IHC (Paraffin sections) (IHC-P). GTX108874

GTX108874 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using POLR2G(GTX108874) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-POLR2G antibody used in Western Blot (WB). GTX108874

GTX108874 WB Image

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
GTX108874 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169