GeneTex
United States (US)

POLR3A antibody

GTX106241_41843_20150612_IP_19011506_116.jpg
GTX106241_39932_IFA_19011506_583.jpg
GTX106241_42123_20150618_WB_19011506_886.jpg

Cat No. GTX106241

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

IMR32 , SK-N-AS

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rna Polymerase Iii Subunit A,Addh,Hld7,Rpc1,Rpc155,Hrpc155,Polr3A

Cellular Localization

Nucleus

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Database

Research Area

DATA IMAGES
GTX106241_41843_20150612_IP_19011506_116.jpg

GTX106241 IP Image

Immunoprecipitation of POLR3A protein from SK-N-SH whole cell extracts using 5 μg of POLR3A antibody (GTX106241).
Western blot analysis was performed using POLR3A antibody (GTX106241).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106241_39932_IFA_19011506_583.jpg

GTX106241 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A(GTX106241) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX106241_42123_20150618_WB_19011506_886.jpg

GTX106241 WB Image

POLR3A antibody detects POLR3A protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody (GTX106241) diluted at a dilution of 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169