Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody (GTX106241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody (GTX106241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody (GTX106241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A(GTX106241) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunoprecipitation of POLR3A protein from SK-N-SH whole cell extracts using 5 μg of POLR3A antibody (GTX106241).
Western blot analysis was performed using POLR3A antibody (GTX106241).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.