GeneTex
United States (US)

POLR3F antibody

GTX105818_40744_WB_19011506_839.jpg

Cat No. GTX105818

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human POLR3F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC13517 antibody, RPC39 antibody, RPC6 antibody, POLR3F antibody, DNA-directed RNA polymerase III subunit F antibody, DNA-directed RNA polymerase III subunit RPC6 antibody, DNA-directed RNA polymerases III 39 kDa polypeptide antibody, RNA polymerase III C39 subunit antibody, DNA-directed RNA polymerase III 39 kDa polypeptide antibody, RNA polymerase III subunit C6 antibody, RNA polymerase III 39 kDa subunit antibody, "polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa antibody"

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105818_40744_WB_19011506_839.jpg

GTX105818 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
10% SDS PAGE
GTX105818 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf