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POM121 antibody [N2N3]

Orthogonal
Anti-POM121 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX102128
Anti-POM121 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX102128
Anti-POM121 antibody [N2N3] used in Western Blot (WB). GTX102128
Anti-POM121 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102128
Anti-POM121 antibody [N2N3] used in Western Blot (WB). GTX102128

Cat. No. GTX102128

Reference 14

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all POM121 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POM121. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POM121 transmembrane nucleoporin , P145 , POM121A

Cellular Localization

Nucleus , nuclear pore complex , Nucleus membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass membrane protein

Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-POM121 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX102128

GTX102128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POM121 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX102128

GTX102128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POM121 antibody [N2N3] used in Western Blot (WB). GTX102128

GTX102128 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] (GTX102128) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-POM121 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102128

GTX102128 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using POM121(GTX102128) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11305 (Red) at 1:500.

Anti-POM121 antibody [N2N3] used in Western Blot (WB). GTX102128

GTX102128 WB Image

Orthogonal

The data was published in the journal Acta Neuropathol in 2016. PMID: 26839082

REFERENCE

Application Reference

Liu S et al. Cell Rep Med 2024; 5 (2) : 101381 UCHL1 is a potential molecular indicator and therapeutic target for neuroendocrine carcinomas.
Xue G et al. Nat Commun 2023; 14 (1) : 3782 The HIV-1 capsid core is an opportunistic nuclear import receptor.
LaJoie D et al. Mol Biol Cell 2022; 33 (13) : ar117 A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.
Application : ICC/IF
Reactivity : Human
Andronov L et al. Commun Biol 2022; 5 (1) : 1100 splitSMLM, a spectral demixing method for high-precision multi-color localization microscopy applied to nuclear pore complexes.
Marco S et al. Nat Commun 2021; 12 (1) : 6829 Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Liu S et al. Cell Rep Med 2024; 5 (2):101381 UCHL1 is a potential molecular indicator and therapeutic target for neuroendocrine carcinomas.
Xue G et al. Nat Commun 2023; 14 (1):3782 The HIV-1 capsid core is an opportunistic nuclear import receptor.
LaJoie D et al. Mol Biol Cell 2022; 33 (13):ar117 A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.
Application : ICC/IF
Reactivity : Human
Andronov L et al. Commun Biol 2022; 5 (1):1100 splitSMLM, a spectral demixing method for high-precision multi-color localization microscopy applied to nuclear pore complexes.
Marco S et al. Nat Commun 2021; 12 (1):6829 Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Application : WB
Reactivity : Human
Agote-Aran A et al. EMBO J 2020; 39 (20):e104467 Spatial control of nucleoporin condensation by fragile X-related proteins.
Hoffman LM et al. Mol Biol Cell 2020;:mbcE19010027 Mechanical stress triggers nuclear remodeling and the formation of Transmembrane Actin Nuclear lines with associated Nuclear Pore Complexes.
Reactivity : Mouse
Ma H et al. J Cancer 2019; 10 (19):4473-4480 POM121 is identified as a novel prognostic marker of oral squamous cell carcinoma.
McCloskey A et al. Genes Dev 2018; 32 (19-20):1321-1331 Tpr regulates the total number of nuclear pore complexes per cell nucleus.
Application : ICC/IF
Reactivity : Human
Rodriguez-Bravo V et al. Cell 2018; (Epub) Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import.
Application : WB
Reactivity : Human
Jiang P et al. Acta Neuropathol 2016; 132 (1):77-91 Proaggregant nuclear factor(s) trigger rapid formation of α-synuclein aggregates in apoptotic neurons.
Application : WB
Reactivity : Human
Franks TM et al. Genes Dev 2016; 30 (10):1155-71 Evolution of a transcriptional regulator from a transmembrane nucleoporin.
Application : WB
Reactivity : Human
Schwartz M et al. Nucleus 2015; 6 (1):40-54 Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin.
Application : ICC/IF
Reactivity : Human
Chow KH et al. Mol Biol Cell 2014; 25 (1):160-8 The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function.
Application : WB, ICC/IF
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169