GeneTex
United States (US)

POM121 antibody [N2N3]

GTX102128_42515_20170525_WB_19011506_758.jpg
GTX102128_40401_IHC_M_19011506_507.jpg
GTX102128_40401_IFA_19011506_918.jpg
GTX102128_40401_IHC_19011506_676.jpg

Cat No. GTX102128

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POM121. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pom121 Transmembrane Nucleoporin,P145,Pom121A,Pom121

Cellular Localization

Nucleus , nuclear pore complex , Nucleus membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass membrane protein

Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Database

DATA IMAGES
GTX102128_42515_20170525_WB_19011506_758.jpg

GTX102128 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] (GTX102128) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102128_40401_IHC_M_19011506_507.jpg

GTX102128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102128_40401_IFA_19011506_918.jpg

GTX102128 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using POM121(GTX102128) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11305 (Red) at 1:500.

GTX102128_40401_IHC_19011506_676.jpg

GTX102128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169