GeneTex
United States (US)

PON2 antibody, Internal

GTX88137_2480_WB_18122102_230.jpg
GTX88137_1039_IHC-P_18122102_693.jpg

Cat No. GTX88137

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-0.6μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88137-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2; NP_001018171.1).

Predict Reactivity

Rat, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EKPRARELRIS, from the internal region of the protein sequence according to NP_000296.2; NP_001018171.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Paraoxonase 2,Pon2

Background

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88137_2480_WB_18122102_230.jpg

GTX88137 WB Image

WB analysis of HepG2 lysate using GTX88137 PON2 antibody, Internal.
Dilution : 0.2µg/ml
Loading : 35µg protein in RIPA buffer

GTX88137_1039_IHC-P_18122102_693.jpg

GTX88137 IHC-P Image

IHC-P analysis of human placenta using GTX88137 PON2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

Package List Price ($)
$ 329