GeneTex
United States (US)

PON2 antibody [N1C2]

GTX104061_39876_20151223_IHC-P_19011506_317.jpg
GTX104061_WB_19011506_274.jpg
GTX104061_IHC_19011506_278.jpg
GTX104061_39876_WB_M_liver_19011506_300.jpg

Cat No. GTX104061

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , mouse liver

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PON2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PON2 antibody, paraoxonase nirs antibody, PON 2 antibody, aromatic esterase 2 antibody, A-esterase 2 antibody, serum aryldialkylphosphatase 2 antibody, serum paraoxonase/arylesterase 2 antibody, paraoxonase 2 antibody

Cellular Localization

Membrane; Peripheral membrane protein

Background

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104061_39876_20151223_IHC-P_19011506_317.jpg

GTX104061 IHC-P Image

PON2 antibody [N1C2] detects PON2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PON2 antibody [N1C2] (GTX104061) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104061_WB_19011506_274.jpg

GTX104061 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
PON2 antibody
GTX104061 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104061_IHC_19011506_278.jpg

GTX104061 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PON2(GTX104061) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104061_39876_WB_M_liver_19011506_300.jpg

GTX104061 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX104061 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169