APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , H1299 , HeLa , HepG2 , mouse liver
Predict Reactivity
Rat, Sheep, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PON2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
PON2 antibody, paraoxonase nirs antibody, PON 2 antibody, aromatic esterase 2 antibody, A-esterase 2 antibody, serum aryldialkylphosphatase 2 antibody, serum paraoxonase/arylesterase 2 antibody, paraoxonase 2 antibody
Cellular Localization
Membrane; Peripheral membrane protein
Background
This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX104061 IHC-P Image
PON2 antibody [N1C2] detects PON2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PON2 antibody [N1C2] (GTX104061) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104061 WB Image
Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
PON2 antibody
GTX104061 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104061 IHC-P Image
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PON2(GTX104061) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104061 WB Image
Sample (50 µg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX104061 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
