APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , Mouse liver , HeLa
Predict Reactivity
Rat, Rabbit, Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.73 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human POR. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951323
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome p450 oxidoreductase , CPR , CYPOR , P450R
Cellular Localization
Endoplasmic reticulum membrane; Peripheral membrane protein
Background
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101](/templates/default/js/lightbox/images/loading.gif "Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101")
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GTX101101 WB Image
Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [C2C3], C-term (GTX101101) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX101101 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX101101 diluted at 1:1000
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GTX101101 IHC-P Image
POR antibody [C2C3], C-term detects POR protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
POR antibody [C2C3], C-term (GTX101101) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101101 WB Image
Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX101101 diluted at 1:2000
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REFERENCE
There are currently no references for POR antibody [C2C3], C-term (GTX101101). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for POR antibody [C2C3], C-term (GTX101101). Be the first to share your experience with this product.
![Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101](/upload/website/prouct_img/small/GTX101101/GTX101101_40079_20170323_WB_KO_watermark_w_23060100_388.webp "Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101")
![Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101](/upload/website/prouct_img/small/GTX101101/GTX101101_40079_WB_M_Liver_w_23060100_865.webp "Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101")
![Anti-POR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101101](/upload/website/prouct_img/small/GTX101101/GTX101101_40079_IHC_w_23060100_255.webp "Anti-POR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101101")
![Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101](/upload/website/prouct_img/small/GTX101101/GTX101101_40079_WB_w_23060100_196.webp "Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101")
![Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101](/upload/website/prouct_img/normal/GTX101101/GTX101101_40079_20170323_WB_KO_watermark_w_23060100_388.webp "Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101")
![Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101](/upload/website/prouct_img/normal/GTX101101/GTX101101_40079_WB_M_Liver_w_23060100_865.webp "Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101")
![Anti-POR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101101](/upload/website/prouct_img/normal/GTX101101/GTX101101_40079_IHC_w_23060100_255.webp "Anti-POR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101101")
![Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101](/upload/website/prouct_img/normal/GTX101101/GTX101101_40079_WB_w_23060100_196.webp "Anti-POR antibody [C2C3], C-term used in Western Blot (WB). GTX101101")