Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [C2C3], C-term (GTX101101) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX101101 diluted at 1:2000

POR antibody [C2C3], C-term detects POR protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
POR antibody [C2C3], C-term (GTX101101) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX101101 diluted at 1:1000