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POR antibody [N1N3]

Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099
Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099
Anti-POR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101099
Anti-POR antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101099

Cat No. GTX101099

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HeLa , mouse lung

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cytochrome p450 oxidoreductase,CPR,CYPOR,P450R

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099

GTX101099 WB Image

Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N1N3] (GTX101099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099

GTX101099 WB Image

POR antibody detects POR protein by western blot analysis.
A. 50 µg mouse lung lysate/extract
7.5% SDS-PAGE
POR antibody (GTX101099) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-POR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101099

GTX101099 IHC-P Image

POR antibody detects POR protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
POR antibody (GTX101099) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POR antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101099

GTX101099 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytochrome P450 Reductase(GTX101099) antibody at 1:200 dilution.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169