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POR antibody [N1N3]

KO/KD
Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099
Anti-POR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101099
Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099
Anti-POR antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101099

Cat. No. GTX101099

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cytochrome P450 Reductase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

mouse lung , HeLa

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome p450 oxidoreductase , CPR , CYPOR , P450R

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099

GTX101099 WB Image

KO/KD

Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N1N3] (GTX101099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-POR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101099

GTX101099 IHC-P Image

POR antibody detects POR protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
POR antibody (GTX101099) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-POR antibody [N1N3] used in Western Blot (WB). GTX101099

GTX101099 WB Image

POR antibody detects POR protein by western blot analysis.
A. 50 μg mouse lung lysate/extract
7.5% SDS-PAGE
POR antibody (GTX101099) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-POR antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101099

GTX101099 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytochrome P450 Reductase(GTX101099) antibody at 1:200 dilution.

REFERENCE

Application Reference

Campelo D et al. Int J Mol Sci 2018; 19 (12) Probing the Role of the Hinge Segment of Cytochrome P450 Oxidoreductase in the Interaction with Cytochrome P450.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169