*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa , HeLaS3 , HepG2
Mouse, Rat, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human POR. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
cytochrome p450 oxidoreductase , CPR , CYPOR , P450R
Endoplasmic reticulum membrane; Peripheral membrane protein
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]
GTX101100 WB Image
Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N2C2], Internal (GTX101100) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101100 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101100 diluted at 1:1000
GTX101100 IHC-P Image
POR antibody [N2C2], Internal detects Cytochrome P450 Reductase protein at endoplasmic reticulum on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
POR antibody [N2C2], Internal (GTX101100) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min