GeneTex
United States (US)

POR antibody [N2C2], Internal

KO/KD
GTX101100_39869_20170323_WB_KO_watermark_19011506_638.jpg
GTX101100_WB_19011506_201.jpg
GTX101100_IHC_19011506_239.jpg

Cat No. GTX101100

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HeLa , HeLaS3 , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human POR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CPR antibody, CYPOR antibody, DKFZp686G04235 antibody, FLJ26468 antibody, P450R antibody, POR antibody, NADPH--cytochrome P450 reductase antibody, NADPH-dependent cytochrome P450 reductase antibody, P450 (cytochrome) oxidoreductase antibody

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101100_39869_20170323_WB_KO_watermark_19011506_638.jpg

GTX101100 WB Image

KO/KD

Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N2C2], Internal (GTX101100) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101100_WB_19011506_201.jpg

GTX101100 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101100 diluted at 1:1000

GTX101100_IHC_19011506_239.jpg

GTX101100 IHC-P Image

POR antibody [N2C2], Internal detects Cytochrome P450 Reductase protein at endoplasmic reticulum on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
POR antibody [N2C2], Internal (GTX101100) dilution: 1:5
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169