Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N2C2], Internal (GTX101100) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

POR antibody [N2C2], Internal detects Cytochrome P450 Reductase protein at endoplasmic reticulum on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
POR antibody [N2C2], Internal (GTX101100) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101100 diluted at 1:1000