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POR antibody

KO/KD
Anti-POR antibody used in Western Blot (WB). GTX32801
Anti-POR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32801
Anti-POR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32801
Anti-POR antibody used in IHC (Paraffin sections) (IHC-P). GTX32801
Anti-POR antibody used in Western Blot (WB). GTX32801
Anti-POR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32801

Cat. No. GTX32801

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Cytochrome P450 Reductase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome p450 oxidoreductase , CPR , CYPOR , P450R

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-POR antibody used in Western Blot (WB). GTX32801

GTX32801 WB Image

WB analysis of various sample lysates using GTX32801 POR antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32801

GTX32801 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32801 POR antibody.
Blue : DAPI
Dilution : 1:100

Anti-POR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32801

GTX32801 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32801 POR antibody.
Blue : DAPI
Dilution : 1:100

Anti-POR antibody used in IHC (Paraffin sections) (IHC-P). GTX32801

GTX32801 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32801 POR antibody.
Dilution : 1:100

Anti-POR antibody used in Western Blot (WB). GTX32801

GTX32801 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32801 POR antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32801

GTX32801 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32801 POR antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399