APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2
PROPERTIES
Form
Liquid
Buffer
1x PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human PORCN
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
porcupine O-acyltransferase , DHOF , FODH , MG61 , PORC , PPN
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Aug 2013]
Database
Research Area
DATA IMAGES
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GTX46000 WB Image
WB analysis of HepG2 cells using GTX46000 PORCN antibody at 0.2-1μg/ml.
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GTX46000 IHC-Fr Image
IHC-Fr analysis of mouse uterus tissue using GTX46000 PORCN antibody at 1:300.
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GTX46000 WB Image
WB analysis of HeLa cells using GTX46000 PORCN antibody at 1μg/ml.
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REFERENCE
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REVIEW
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