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PORCN antibody, N-term

Anti-PORCN antibody, N-term used in Western Blot (WB). GTX46000
Anti-PORCN antibody, N-term used in IHC (Frozen sections) (IHC-Fr). GTX46000
Anti-PORCN antibody, N-term used in Western Blot (WB). GTX46000

Cat. No. GTX46000

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse
Package
50 μg ($409)
See all PORCN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1x PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human PORCN

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

porcupine O-acyltransferase , DHOF , FODH , MG61 , PORC , PPN

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-PORCN antibody, N-term used in Western Blot (WB). GTX46000

GTX46000 WB Image

WB analysis of HepG2 cells using GTX46000 PORCN antibody at 0.2-1μg/ml.

Anti-PORCN antibody, N-term used in IHC (Frozen sections) (IHC-Fr). GTX46000

GTX46000 IHC-Fr Image

IHC-Fr analysis of mouse uterus tissue using GTX46000 PORCN antibody at 1:300.

Anti-PORCN antibody, N-term used in Western Blot (WB). GTX46000

GTX46000 WB Image

WB analysis of HeLa cells using GTX46000 PORCN antibody at 1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409