GeneTex
United States (US)

POU2F3 antibody, Internal

GTX88114_2521_WB_18122102_738.jpg

Cat No. GTX88114

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88114-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EPHLEASQHLPVPKH, from the internal region of the protein sequence according to NP_055167.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pou Class 2 Homeobox 3,Epoc-1,Oct-11,Oct11,Otf-11,Pla-1,Pla1,Skn-1A,Pou2F3

Background

This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Database

DATA IMAGES
GTX88114_2521_WB_18122102_738.jpg

GTX88114 WB Image

WB analysis of HEK293 lysate using GTX88114 POU2F3 antibody, Internal.
Dilution : 2µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329