APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Dog, Guinea pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 2% sucrose
Preservative
0.09% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human POU2F3.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
POU class 2 homeobox 3 , Epoc-1 , OCT-11 , OCT11 , OTF-11 , PLA-1 , PLA1 , Skn-1a
Cellular Localization
Nucleus
Background
This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
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GTX04103 IHC Image
IHC analysis of human muscle tissue using GTX04103 POU2F3 antibody.
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GTX04103 IHC-P Image
IHC-P analysis of human skin tissue using GTX04103 POU2F3 antibody. Dilution : 1:200
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GTX04103 IHC Image
IHC analysis of mouse tongue tissue using GTX04103 POU2F3 antibody. Dilution : 1:100
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GTX04103 WB Image
WB analysis of various sample lysates using GTX04103 POU2F3 antibody. Dilution : 0.5μg/ml Loading : 25μg per lane
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GTX04103 WB Image
WB analysis of rat brain tissue lysate using GTX04103 POU2F3 antibody. Dilution : 1μg/ml
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REFERENCE
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REVIEW
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