APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88096-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).
Predict Reactivity
Human, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence RSEMNAELRGEDK-C, from the N Terminus of the protein sequence according to NP_009183.3; NP_001159490.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
POU class 6 homeobox 2 , RPF-1 , WT5 , WTSL
Cellular Localization
Nucleus
Background
This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
|
GTX88096 WB Image
WB analysis of mouse eye lysate using GTX88096 POU6F2 antibody, N-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for POU6F2 antibody, N-term (GTX88096). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for POU6F2 antibody, N-term (GTX88096). Be the first to share your experience with this product.