GeneTex
United States (US)

POU6F2 antibody, N-term

GTX88096_2524_WB_18121411_401.jpg

Cat No. GTX88096

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88096-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).

Predict Reactivity

Human, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RSEMNAELRGEDK-C, from the N Terminus of the protein sequence according to NP_009183.3; NP_001159490.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pou Class 6 Homeobox 2,Rpf-1,Wt5,Wtsl,Pou6F2

Background

This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX88096_2524_WB_18121411_401.jpg

GTX88096 WB Image

WB analysis of mouse eye lysate using GTX88096 POU6F2 antibody, N-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer