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PP1C gamma antibody [N1C2]

Orthogonal
Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618
Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618
Anti-PP1C gamma antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105618
Anti-PP1C gamma antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105618
Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618

Cat. No. GTX105618

Reference 3
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PP1C gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

NIH-3T3

Product Note

Based on sequence homology, this antibody may cross react with PPP1CA or PPP1CB proteins.

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PP1C gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951350

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 catalytic subunit gamma , PP-1G , PP1C , PPP1G

Background

Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.

Database

Research Area

DATA IMAGES

Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618

GTX105618 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP1C gamma antibody [N1C2] (GTX105618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618

GTX105618 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3 10% SDS PAGE
GTX105618 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP1C gamma antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105618

GTX105618 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP1 gamma(GTX105618) antibody at 1:200 dilution.

Anti-PP1C gamma antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105618

GTX105618 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1CC(GTX105618) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618

GTX105618 WB Image

Orthogonal

The data was published in the journal J Neurochem in 2016.PMID: 26485687

REFERENCE

Application Reference

Ueda T et al. Cell Signal 2019; 55 : 81-89 IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.
Application : WB
Reactivity : Human
Kenney JW et al. J Neurochem 2016; 136 (2) : 276-84 Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons.
Application : WB
Wei R et al. Mol Biol Cell 2011; 22 (19) : 3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

PP1C gamma antibody [N1C2] Cat. No. GTX105618

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618
Date : Anonymous submitted on 25-Jun-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 and HeLa cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:3000, 25°C, 2Hr
Lane Description : Lane 1: HEK293 cells; Lane 2: HeLa cells
Anti-PP1C gamma antibody [N1C2] used in Western Blot (WB). GTX105618
Date : Anonymous submitted on 04-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : KATO III cells and SCM1 cells
Amount used : 50μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane 1: KATO III cells, Lane 2: SCM1 cells.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169