APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PP2A alpha + PP2A beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887068
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
PP2Ac antibody , PP2CA antibody , PP2Calpha antibody , PPP2CA antibody , RP C antibody , protein phosphatase 2 catalytic subunit alpha antibody
Background
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Research Area
DATA IMAGES
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GTX133709 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PP2A alpha + PP2A beta antibody (GTX133709) diluted at 1:1000 and competitor's antibody (sc-14020) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX133709 IHC-P Image
PP2A alpha + PP2A beta antibody detects PP2A alpha + PP2A beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.PP2A alpha + PP2A beta stained by PP2A alpha + PP2A beta antibody (GTX133709) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133709 IHC-P Image
PP2A alpha + PP2A beta antibody detects PP2A alpha + PP2A beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.PP2A alpha + PP2A beta stained by PP2A alpha + PP2A beta antibody (GTX133709) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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