APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PP2A alpha + PP2A beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887068
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
PP2Ac antibody , PP2CA antibody , PP2Calpha antibody , PPP2CA antibody , RP C antibody , protein phosphatase 2 catalytic subunit alpha antibody
Background
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Research Area
DATA IMAGES
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GTX133709 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PP2A alpha + PP2A beta antibody (GTX133709) diluted at 1:1000 and competitor's antibody (sc-14020) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX133709 IHC-P Image
PP2A alpha + PP2A beta antibody detects PP2A alpha + PP2A beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.PP2A alpha + PP2A beta stained by PP2A alpha + PP2A beta antibody (GTX133709) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133709 IHC-P Image
PP2A alpha + PP2A beta antibody detects PP2A alpha + PP2A beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.PP2A alpha + PP2A beta stained by PP2A alpha + PP2A beta antibody (GTX133709) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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