Non-transfected (–) and transfected (+) PP2A alpha shRNA-transfected 293T (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PP2A alpha antibody [HL1447] detects PP2A alpha protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).