GeneTex

  • Country / Location Selection

United States (US)

PP2A alpha antibody [HL1447]

Recombinant antibody KO/KD
Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919
Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919
Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919
Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919
Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919
Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919
Anti-PP2A alpha antibody [HL1447] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636919
Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919
Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919

Cat. No. GTX636919

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1447

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all PP2A alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 2 catalytic subunit alpha , PP2Ac , PP2CA , PP2Calpha , RP-C

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919

GTX636919 WB Image

KO/KD

Non-transfected (–) and transfected (+) PP2A alpha shRNA-transfected 293T (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919

GTX636919 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919

GTX636919 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919

GTX636919 IHC-P Image

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919

GTX636919 IHC-P Image

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PP2A alpha antibody [HL1447] used in Western Blot (WB). GTX636919

GTX636919 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP2A alpha antibody [HL1447] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636919

GTX636919 ICC/IF Image

PP2A alpha antibody [HL1447] detects PP2A alpha protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919

GTX636919 IHC-P Image

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PP2A alpha antibody [HL1447] used in IHC (Paraffin sections) (IHC-P). GTX636919

GTX636919 IHC-P Image

PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
PP2A alpha stained by PP2A alpha antibody [HL1447] (GTX636919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for PP2A alpha antibody [HL1447] (GTX636919). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PP2A alpha antibody [HL1447] (GTX636919). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations