PP2A alpha antibody

Name: PP2A alpha antibody
Brand: GeneTex
SKU: GTX106334
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-PP2A alpha antibody used in Western Blot (WB). GTX106334

Anti-PP2A alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106334

Anti-PP2A alpha antibody used in Immunoprecipitation (IP). GTX106334

Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX106334

Cat No. GTX106334

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all PP2A alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241211

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 2 catalytic subunit alpha , PP2Ac , PP2CA , PP2Calpha , RP-C

Cellular Localization

Cytoplasm , Nucleus , Centromere , Spindle

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq]

Database

Gene ID: 5515 PPP2CA
UniProt: P67775 PPP2CA

Research Area

DATA IMAGES

GTX106334 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX106334) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106334 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PP2A alpha(GTX106334) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX106334 IP Image

Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 μg of PP2A alpha antibody (GTX106334).
Western blot analysis was performed using PP2A alpha antibody (GTX106334).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106334 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX106334 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106334 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PPP2CA (GTX106334) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106334 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX106334 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kobayashi Y et al. Biomedicines 2021; 9 (7) Omalizumab Restores Response to Corticosteroids in Patients with Eosinophilic Chronic Rhinosinusitis and Severe Asthma.

Application : ICC/IF

Reactivity : Human

Di Domenico F et al. Redox Biol 2019; 23 : 101162 Restoration of aberrant mTOR signaling by intranasal rapamycin reduces oxidative damage: Focus on HNE-modified proteins in a mouse model of down syndrome.

Sheu JR et al. J Cell Mol Med 2017; (Epub) CME-1, a novel polysaccharide, suppresses iNOS expression in lipopolysaccharide-stimulated macrophages through ceramide-initiated protein phosphatase 2A activation.

Zhang W et al. RSC Adv 2016; 6 : 48072-48082 HDACI regulates the PI3K/Akt signaling pathway to reverse MCF-7/PTX resistance by inhibiting SET.

Application : WB

Reactivity : Human

Hsieh CY et al. J Cell Mol Med 2014; PMC, a potent hydrophilic α-tocopherol derivative, inhibits NF-κB activation via PP2A but not IκBα-dependent signals in vascular smooth muscle cells.

Application : WB

Reactivity : Rat

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