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PP2A alpha antibody

KO/KD
Anti-PP2A alpha antibody used in Western Blot (WB). GTX106334
Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX106334
Anti-PP2A alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106334
Anti-PP2A alpha antibody used in Immunoprecipitation (IP). GTX106334
Anti-PP2A alpha antibody used in Western Blot (WB). GTX106334
Anti-PP2A alpha antibody used in Western Blot (WB). GTX106334
Anti-PP2A alpha antibody used in Western Blot and Immunoprecipitation (WB IP). GTX106334

Cat. No. GTX106334

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PP2A alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , Molt-4 , Mouse brain , 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241211

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 2 catalytic subunit alpha , PP2Ac , PP2CA , PP2Calpha , RP-C

Cellular Localization

Cytoplasm , Nucleus , Centromere , Spindle

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PP2A alpha antibody used in Western Blot (WB). GTX106334

GTX106334 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX106334) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX106334

GTX106334 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PPP2CA (GTX106334) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PP2A alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106334

GTX106334 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PP2A alpha(GTX106334) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PP2A alpha antibody used in Immunoprecipitation (IP). GTX106334

GTX106334 IP Image

Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 μg of PP2A alpha antibody (GTX106334).
Western blot analysis was performed using PP2A alpha antibody (GTX106334).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PP2A alpha antibody used in Western Blot (WB). GTX106334

GTX106334 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX106334 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP2A alpha antibody used in Western Blot (WB). GTX106334

GTX106334 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX106334 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PP2A alpha antibody used in Western Blot and Immunoprecipitation (WB IP). GTX106334

GTX106334 WB IP Image

The data was published in the journal J Cell Mol Med in 2014. PMID: 24725826

REFERENCE

Application Reference

Ripamonti M et al. Commun Biol 2022; 5 (1) : 1025 A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility.
Kobayashi Y et al. Biomedicines 2021; 9 (7) Omalizumab Restores Response to Corticosteroids in Patients with Eosinophilic Chronic Rhinosinusitis and Severe Asthma.
Application : ICC/IF
Reactivity : Human
Di Domenico F et al. Redox Biol 2019; 23 : 101162 Restoration of aberrant mTOR signaling by intranasal rapamycin reduces oxidative damage: Focus on HNE-modified proteins in a mouse model of down syndrome.
Sheu JR et al. J Cell Mol Med 2017; (Epub) CME-1, a novel polysaccharide, suppresses iNOS expression in lipopolysaccharide-stimulated macrophages through ceramide-initiated protein phosphatase 2A activation.
Zhang W et al. RSC Adv 2016; 6 : 48072-48082 HDACI regulates the PI3K/Akt signaling pathway to reverse MCF-7/PTX resistance by inhibiting SET.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169