GeneTex
United States (US)

PP2A alpha antibody

KO/KD
GTX106334_39708_20160804_WB_shRNA_watermark_19011506_136.jpg
GTX106334_40394_IFA_19011506_519.jpg
GTX106334_39708_20150625_IP_19011506_787.jpg
GTX106334_39708_WB_M_brain_19011506_379.jpg
GTX106334_IHC_19011506_154.jpg
GTX106334_WB_19011506_583.jpg

Cat No. GTX106334

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.479mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PP2Ac antibody, PP2CA antibody, PP2Calpha antibody, RP-C antibody, PPP2CA antibody, PP2A-alpha antibody, replication protein C antibody, "serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform antibody", "protein phosphatase 2A catalytic subunit, alpha isoform antibody", serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody, "protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform antibody", "protein phosphatase 2, catalytic subunit, alpha isozyme antibody"

Cellular Localization

Cytoplasm , Nucleus , Centromere , Spindle

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106334_39708_20160804_WB_shRNA_watermark_19011506_136.jpg

GTX106334 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX106334) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106334_40394_IFA_19011506_519.jpg

GTX106334 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PP2A alpha(GTX106334) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX106334_39708_20150625_IP_19011506_787.jpg

GTX106334 IP Image

Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 μg of PP2A alpha antibody (GTX106334).
Western blot analysis was performed using PP2A alpha antibody (GTX106334).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106334_39708_WB_M_brain_19011506_379.jpg

GTX106334 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX106334 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106334_IHC_19011506_154.jpg

GTX106334 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PPP2CA (GTX106334) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106334_WB_19011506_583.jpg

GTX106334 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX106334 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf