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PP2A alpha antibody

Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113523
Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113523
Anti-PP2A alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113523
Anti-PP2A alpha antibody used in Western Blot (WB). GTX113523
Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113523
Anti-PP2A alpha antibody used in Western Blot (WB). GTX113523

Cat No. GTX113523

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HCT116 , HeLa , HepG2 , Molt-4 , mouse brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PP2A alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037767

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 2 catalytic subunit alpha , PP2Ac , PP2CA , PP2Calpha , RP-C

Cellular Localization

Cytoplasm , Nucleus , Centromere , Spindle

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113523

GTX113523 IHC-P Image

PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PP2A alpha stained by PP2A alpha antibody (GTX113523) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113523

GTX113523 IHC-P Image

PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PP2A alpha stained by PP2A alpha antibody (GTX113523) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PP2A alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113523

GTX113523 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PP2A alpha(GTX113523) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PP2A alpha antibody used in Western Blot (WB). GTX113523

GTX113523 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113523 diluted at 1:1000

Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113523

GTX113523 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PP2A(GTX113523) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PP2A alpha antibody used in Western Blot (WB). GTX113523

GTX113523 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX113523) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

PP2A alpha antibody Cat No. GTX113523

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-Dec-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 and HeLa cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TTBS, 25°C, 0.5Hr
Primary Antibody : 1:3000, 4°C, 12Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169