PP2A alpha antibody

Name: PP2A alpha antibody
Brand: GeneTex
SKU: GTX113523
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-PP2A alpha antibody used in Western Blot (WB). GTX113523

Anti-PP2A alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113523

Anti-PP2A alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113523

Cat. No. GTX113523

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all PP2A alpha products

View product citations for antibody GTX113523 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PP2A alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037767

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 2 catalytic subunit alpha , PP2Ac , PP2CA , PP2Calpha , RP-C

Cellular Localization

Cytoplasm , Nucleus , Centromere , Spindle

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq]

Database

Gene ID: 5515 PPP2CA
UniProt: P67775 PPP2CA

Research Area

DATA IMAGES

GTX113523 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX113523) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113523 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113523 diluted at 1:1000

GTX113523 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PP2A(GTX113523) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113523 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PP2A alpha(GTX113523) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX113523 IHC-P Image

PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PP2A alpha stained by PP2A alpha antibody (GTX113523) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113523 IHC-P Image

PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PP2A alpha stained by PP2A alpha antibody (GTX113523) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ueda T et al. Cell Signal 2019; 55 : 81-89 IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.

Application : WB

Reactivity : Human

Chen YY et al. Sci Rep 2014; 4 : 5651 Andrographolide induces vascular smooth muscle cell apoptosis through a SHP-1-PP2A-p38MAPK-p53 cascade.

Application : WB

Submit a Reference

REVIEW