APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HCT116 , HeLa , HepG2 , Molt-4 , mouse brain
Predict Reactivity
Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PP2A alpha. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037767
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
protein phosphatase 2 catalytic subunit alpha , PP2Ac , PP2CA , PP2Calpha , RP-C
Cellular Localization
Cytoplasm , Nucleus , Centromere , Spindle
Background
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113523 IHC-P Image
PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.PP2A alpha stained by PP2A alpha antibody (GTX113523) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113523 IHC-P Image
PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.PP2A alpha stained by PP2A alpha antibody (GTX113523) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113523 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PP2A alpha(GTX113523) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX113523 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113523 diluted at 1:1000
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GTX113523 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PP2A(GTX113523) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113523 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX113523) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
PP2A alpha antibody
Cat No. GTX113523
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 26-Dec-2019
Application Tested :
WB
Sample Species :
Hu
Sample :
HEK293 and HeLa cells
Amount used :
20 μg
Blocking :
5% non-fat milk in TTBS, 25°C, 0.5Hr
Primary Antibody :
1:3000, 4°C, 12Hr
