APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Rat, Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886269
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
protein phosphatase 2 catalytic subunit alpha , PP2Ac , PP2CA , PP2Calpha , RP-C
Cellular Localization
Cytoplasm , Nucleus , Centromere , Spindle
Background
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX130434 WB Image
PP2A alpha antibody detects PP2A alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody (GTX130434) diluted at a dilution of 1:1000.
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GTX130434 IP Image
Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 μg of PP2A alpha antibody (GTX130434).
Western blot analysis was performed using PP2A alpha antibody (GTX130434).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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REFERENCE
There are currently no references for PP2A alpha antibody (GTX130434). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PP2A alpha antibody (GTX130434). Be the first to share your experience with this product.
