GeneTex
United States (US)

PP2A beta antibody

Overexpression
GTX101690_39609_20181109_WB_B_19011519_633.jpg
GTX101690_WB_19011506_660.jpg
GTX101690_39609_WB_M_brain_19011506_976.jpg
GTX101690_39609_IFA_19011506_446.jpg

Cat No. GTX101690

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , mouse brain

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PP2A beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PP2Abeta antibody, PP2CB antibody, PPP2CB antibody, "protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody", "protein phosphatase 2, catalytic subunit, beta isoform antibody", "protein phosphatase 2A catalytic subunit, beta isoform antibody", protein phosphatase type 2A catalytic subunit antibody, serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody, "serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform antibody", PP2A-beta antibody, "protein phosphatase 2, catalytic subunit, beta isozyme antibody"

Cellular Localization

Cytoplasm , Nucleus , Centromere , Spindle

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101690_39609_20181109_WB_B_19011519_633.jpg

GTX101690 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A beta antibody (GTX101690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101690_WB_19011506_660.jpg

GTX101690 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
12% SDS PAGE
GTX101690 diluted at 1:500

GTX101690_39609_WB_M_brain_19011506_976.jpg

GTX101690 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101690 diluted at 1:1000

GTX101690_39609_IFA_19011506_446.jpg

GTX101690 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP2A beta(GTX101690) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169