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PPA1 antibody [AT4G4]

Anti-PPA1 antibody [AT4G4] used in Flow cytometry (FACS). GTX57682
Anti-PPA1 antibody [AT4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57682
Anti-PPA1 antibody [AT4G4] used in Western Blot (WB). GTX57682

Cat. No. GTX57682

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT4G4

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all PPA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT4G4 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPA3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inorganic pyrophosphatase 1 , HELS66p , IOPPP , PP , PP1 , PPA1 , SID68061 , pyrophosphatase (inorganic) 1 , Pyrophosphatase 1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPA1 antibody [AT4G4] used in Flow cytometry (FACS). GTX57682

GTX57682 FACS Image

FACS analysis of HeLa cells using GTX57682 PPA1 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-PPA1 antibody [AT4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57682

GTX57682 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57682 PPA1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-PPA1 antibody [AT4G4] used in Western Blot (WB). GTX57682

GTX57682 WB Image

WB analysis of various samples using GTX57682 PPA1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : U87-MG whole cell lysate
Lane 6 : PC-3 whole cell lysate
Lane 7 : WiDr whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399