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PPA1 antibody

Anti-PPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111031
Anti-PPA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111031
Anti-PPA1 antibody used in Western Blot (WB). GTX111031
Anti-PPA1 antibody used in Western Blot (WB). GTX111031
Anti-PPA1 antibody used in Western Blot (WB). GTX111031
Anti-PPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111031

Cat. No. GTX111031

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PPA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951331

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyrophosphatase (inorganic) 1 , HEL-S-66p , IOPPP , PP , PP1 , SID6-8061

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111031

GTX111031 IHC-P Image

PPA1 antibody detects PPA1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PPA1 stained by PPA1 antibody (GTX111031) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111031

GTX111031 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPA1(GTX111031) antibody at 1:200 dilution.

Anti-PPA1 antibody used in Western Blot (WB). GTX111031

GTX111031 WB Image

PPA1 antibody detects PPA1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12% SDS-PAGE
PPA1 antibody (GTX111031) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPA1 antibody used in Western Blot (WB). GTX111031

GTX111031 WB Image

PPA1 antibody detects PPA1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
PPA1 antibody (GTX111031) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPA1 antibody used in Western Blot (WB). GTX111031

GTX111031 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PPA1 antibody (GTX111031) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111031

GTX111031 IHC-P Image

PPA1 antibody detects PPA1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PPA1 antibody (GTX111031) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169