APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2-5 μg/ml |
| 2μg/ml |
| 1μg per 10⁶ cells |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant PPAR alpha protein.
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR
Cellular Localization
Nucleus
Background
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412](/templates/default/js/lightbox/images/loading.gif "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412")
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GTX12412 FACS Image
FACS analysis of Hela cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX12412 WB Image
WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX12412 PPAR alpha antibody [3B6/PPAR].
Dilution : 1:1000 (lane 1 and 2) & 1:500 (lane 3)
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GTX12412 FACS Image
FACS analysis of 3T3 cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX12412 FACS Image
FACS analysis of Jurkat cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX12412 ICC/IF Image
ICC/IF analysis of HCT116 cells using GTX12412 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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REFERENCE
There are currently no references for PPAR alpha antibody [3B6/PPAR] (GTX12412). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PPAR alpha antibody [3B6/PPAR] (GTX12412). Be the first to share your experience with this product.
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412](/upload/website/prouct_img/small/GTX12412/GTX12412_89_FACS_w_23060522_640.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX12412](/upload/website/prouct_img/small/GTX12412/GTX12412_1480_WB_w_23060522_816.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412](/upload/website/prouct_img/small/GTX12412/GTX12412_88_FACS_w_23060522_280.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412](/upload/website/prouct_img/small/GTX12412/GTX12412_90_FACS_w_23060522_233.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12412](/upload/website/prouct_img/small/GTX12412/GTX12412_246_ICC-IF_w_23060522_766.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412](/upload/website/prouct_img/normal/GTX12412/GTX12412_89_FACS_w_23060522_640.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX12412](/upload/website/prouct_img/normal/GTX12412/GTX12412_1480_WB_w_23060522_816.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412](/upload/website/prouct_img/normal/GTX12412/GTX12412_88_FACS_w_23060522_280.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412](/upload/website/prouct_img/normal/GTX12412/GTX12412_90_FACS_w_23060522_233.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12412](/upload/website/prouct_img/normal/GTX12412/GTX12412_246_ICC-IF_w_23060522_766.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12412")