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PPAR alpha antibody [3B6/PPAR]

Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12412
Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412
Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX12412
Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412
Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412

Cat No. GTX12412

Host

Mouse

Clonality

Monoclonal

Clone Name

3B6/PPAR

Isotype

IgG2b

Application

WB, ICC/IF, FACS, IP, ChIP assay, Gel supershift assays

Reactivity

Human, Mouse, Rat, Rabbit, Bovine
Package
50 μg ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2-5 μg/ml
ICC/IF 2μg/ml
FACS 1μg per 10⁶ cells
IP Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant PPAR alpha protein.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR

Cellular Localization

Nucleus

Background

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12412

GTX12412 ICC/IF Image

ICC/IF analysis of HCT116 cells using GTX12412 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412

GTX12412 FACS Image

FACS analysis of Jurkat cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX12412

GTX12412 WB Image

WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX12412 PPAR alpha antibody [3B6/PPAR].
Dilution : 1:1000 (lane 1 and 2) & 1:500 (lane 3)

Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412

GTX12412 FACS Image

FACS analysis of 3T3 cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX12412

GTX12412 FACS Image

FACS analysis of Hela cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

There are currently no references for PPAR alpha antibody [3B6/PPAR] (GTX12412). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PPAR alpha antibody [3B6/PPAR] (GTX12412). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 259