Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant PPAR alpha protein.
Purification
Purified IgG
Conjugation
Unconjugated
RRID
AB_11166646
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
FACS analysis of Hela cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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GTX22779 WB Image
WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX22779 PPAR alpha antibody [3B6/PPAR]. Dilution : 1:1000 (Hela and Jurkat) and 1:500 (NIH-3T3)
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GTX22779 FACS Image
FACS analysis of 3T3 cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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GTX22779 ICC/IF Image
ICC/IF analysis of HCT116 cells using GTX22779 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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GTX22779 FACS Image
FACS analysis of Jurkat cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779](/upload/website/prouct_img/small/GTX22779/GTX22779_139_FACS_w_23060620_153.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX22779](/upload/website/prouct_img/small/GTX22779/GTX22779_1589_WB_w_23060620_302.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779](/upload/website/prouct_img/small/GTX22779/GTX22779_138_FACS_w_23060620_338.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22779](/upload/website/prouct_img/small/GTX22779/GTX22779_432_ICC-IF_w_23060620_463.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779](/upload/website/prouct_img/small/GTX22779/GTX22779_140_FACS_w_23060620_339.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779](/upload/website/prouct_img/normal/GTX22779/GTX22779_139_FACS_w_23060620_153.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX22779](/upload/website/prouct_img/normal/GTX22779/GTX22779_1589_WB_w_23060620_302.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779](/upload/website/prouct_img/normal/GTX22779/GTX22779_138_FACS_w_23060620_338.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22779](/upload/website/prouct_img/normal/GTX22779/GTX22779_432_ICC-IF_w_23060620_463.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22779")
![Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779](/upload/website/prouct_img/normal/GTX22779/GTX22779_140_FACS_w_23060620_339.webp "Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779")