APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:5000-1:10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.01M TBS, 1% BSA, 50% Glycerol
Preservative
0.03% Proclin300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR
Cellular Localization
Nucleus
Background
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX03713 WB Image
WB analysis of various sample lysates using GTX03713 PPAR alpha (phospho Ser12) antibody.
Lane 1 : Mouse Heart
Lane 2 : Mouse Liver
Loading : 40μg
Dilution : 1:300
|
|
|
GTX03713 WB Image
WB analysis of various sample lysates using GTX03713 PPAR alpha (phospho Ser12) antibody.
Lane 1: Mouse Heart lysates
Lane 2 : Mouse Liver lysates
Lane 3 : Mouse Kidney lysates
Lane 4 : Mouse Cerebrum lysates
Lane 5 : Rat Cerebrum lysates
Lane 6 : Jurkat cell lysates
Lane 7 : A431 cell lysates
Dilution : 1:1000
|
|
|
GTX03713 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX03713 PPAR alpha (phospho Ser12) antibody.
Dilution : 1:200
|
|
|
GTX03713 ELISA Image
ELISA analysis of phosphopeptide and non-phosphorylated antigen using serial diluted GTX03713 PPAR alpha (phospho Ser12) antibody.
|
|
|
GTX03713 WB Image
WB analysis of rat liver tissue lysate using GTX03713 PPAR alpha (phospho Ser12) antibody.
Loading : 40μg
Dilution : 1:300
|
REFERENCE
There are currently no references for PPAR alpha (phospho Ser12) antibody (GTX03713). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PPAR alpha (phospho Ser12) antibody (GTX03713). Be the first to share your experience with this product.
