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PPAR alpha (phospho Ser12) antibody

Anti-PPAR alpha (phospho Ser12) antibody used in Western Blot (WB). GTX03713
Anti-PPAR alpha (phospho Ser12) antibody used in ELISA (ELISA). GTX03713
Anti-PPAR alpha (phospho Ser12) antibody used in Western Blot (WB). GTX03713
Anti-PPAR alpha (phospho Ser12) antibody used in IHC (Paraffin sections) (IHC-P). GTX03713
Anti-PPAR alpha (phospho Ser12) antibody used in Western Blot (WB). GTX03713

Cat. No. GTX03713

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:100-1:500
ELISA 1:5000-1:10000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.01M TBS pH7.4, 1% BSA, 50% Glycerol

Preservative

0.03% Proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR

Cellular Localization

Nucleus

Background

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPAR alpha (phospho Ser12) antibody used in Western Blot (WB). GTX03713

GTX03713 WB Image

WB analysis of various sample lysates using GTX03713 PPAR alpha (phospho Ser12) antibody.
Lane 1 : Mouse Heart
Lane 2 : Mouse Liver
Loading : 40μg
Dilution : 1:300

Anti-PPAR alpha (phospho Ser12) antibody used in ELISA (ELISA). GTX03713

GTX03713 ELISA Image

ELISA analysis of phosphopeptide and non-phosphorylated antigen using serial diluted GTX03713 PPAR alpha (phospho Ser12) antibody.

Anti-PPAR alpha (phospho Ser12) antibody used in Western Blot (WB). GTX03713

GTX03713 WB Image

WB analysis of various sample lysates using GTX03713 PPAR alpha (phospho Ser12) antibody.
Lane 1: Mouse Heart lysates
Lane 2 : Mouse Liver lysates
Lane 3 : Mouse Kidney lysates
Lane 4 : Mouse Cerebrum lysates
Lane 5 : Rat Cerebrum lysates
Lane 6 : Jurkat cell lysates
Lane 7 : A431 cell lysates
Dilution : 1:1000

Anti-PPAR alpha (phospho Ser12) antibody used in IHC (Paraffin sections) (IHC-P). GTX03713

GTX03713 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX03713 PPAR alpha (phospho Ser12) antibody.
Dilution : 1:200

Anti-PPAR alpha (phospho Ser12) antibody used in Western Blot (WB). GTX03713

GTX03713 WB Image

WB analysis of rat liver tissue lysate using GTX03713 PPAR alpha (phospho Ser12) antibody.
Loading : 40μg
Dilution : 1:300

REFERENCE

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REVIEW

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Package List Price ($)
$ 399