ICC/IF analysis of 3T3-L1 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

ICC/IF analysis of U-87 MG cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

WB analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates using GTX23484 PPAR alpha (phospho Ser12) antibody.
Dilution : 1:200

ICC/IF analysis of C2C12 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

WB analysis of whole cell extracts (20 μg lysate) of U-87 MG (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), C2C12 (lane 4), Hep G2 (lane 5) and NIH/3T3 (lane 6) using GTX23484 PPAR alpha (phospho Ser12) antibody.
Dilution : 1:250-1:1000