APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89687-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CHPLLQEIYKDMY, from the C Terminus of the protein sequence according to NP_006229.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxisome proliferator activated receptor delta , FAAR , NR1C2 , NUC1 , NUCI , NUCII , PPARB
Cellular Localization
Nucleus
Background
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. The encoded protein is thought to function as an integrator of transcriptional repression and nuclear receptor signaling. It may inhibit the ligand-induced transcriptional activity of peroxisome proliferator activated receptors alpha and gamma, though evidence for this effect is inconsistent. Expression of this gene in colorectal cancer cells may be variable but is typically relatively low. Knockout studies in mice suggested a role for this protein in myelination of the corpus callosum, lipid metabolism, differentiation, and epidermal cell proliferation. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX89687 WB Image
WB analysis of Molt-4 cell lysate using GTX89687 PPAR delta (Isoform 1) antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for PPAR delta (Isoform 1) antibody, C-term (GTX89687). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PPAR delta (Isoform 1) antibody, C-term (GTX89687). Be the first to share your experience with this product.
