WB analysis of mouse heart tissue lysate using GTX28937 PPAR delta antibody.
Loading : 30 μg
Dilution : 1:750

ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX28937 PPAR delta antibody.
Coating : 0.1 μg

Western blotting. Affinity Purified Anti-PPAR delta (Rabbit) is shown to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 μg of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4º C followed reaction with a 1:10,000 dilution of IRDyeR 800 conjugated goat anti-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Rabbit Anti-PPAR delta (N terminal specific) 1:500