ICC/IF analysis of HeLa cells using GTX79186 PPAR delta antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA and incubated for 3 hours at room temperature

WB analysis of whole cell extracts (20 μg lysate) of NIH/3T3 (Lane 1), THP-1 (Lane 2), PC-3 (Lane 3), PC-12 (Lane 4), SH-SY5Y (Lane 5), U 2-OS (Lane 6), HCT 116 (Lane 7), K562 (Lane 8), A-431 (Lane 9) and Jurkat (Lane 10) using GTX79186 PPAR delta antibody.
Dilution : 1:250-1:750

WB analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates using GTX79186 PPAR delta antibody.
Dilution : 1:2000 (NIH-3T3 and K562) and 1:1000 (PC12)