For WB: Use at a dilution of 1:2000-1:5000. Optimal dilutions/concentrations should be determined by the end user.
Human adipose protein lysate
it should react with Human PPAR gamma isoform1 and isoform 2
PBS, pH7.2 with 0.02% sodium azide as preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide - amino acids (YYSEKTQLYNKPHEE) of human PPAR gamma
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Peroxisome Proliferator Activated Receptor Gamma,Cimt1,Glm1,Nr1C3,Pparg1,Pparg2,Ppargamma,Pparg
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARis). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARis are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARis can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha.
GTX30762 WB Image
Western blot analysis of GTX30762 chicken anti PPAR gamma Ab (1:2000) on human adipose protein lysate (50ug)
GTX30762 WB Image