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PPAR gamma antibody

Anti-PPAR gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX32803
Anti-PPAR gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX32803
Anti-PPAR gamma antibody used in Western Blot (WB). GTX32803
Anti-PPAR gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32803
Anti-PPAR gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32803
Anti-PPAR gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX32803
Anti-PPAR gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32803

Cat. No. GTX32803

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PPAR gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PPARG (NP_056953.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887681

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxisome proliferator activated receptor gamma , CIMT1 , GLM1 , NR1C3 , PPARG1 , PPARG2 , PPARgamma

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPAR gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX32803

GTX32803 IHC-P Image

IHC-P analysis of human colon tissue using GTX32803 PPAR gamma antibody.
Dilution : 1:100

Anti-PPAR gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX32803

GTX32803 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX32803 PPAR gamma antibody.
Dilution : 1:100

Anti-PPAR gamma antibody used in Western Blot (WB). GTX32803

GTX32803 WB Image

WB analysis of various sample lysates using GTX32803 PPAR gamma antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PPAR gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32803

GTX32803 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32803 PPAR gamma antibody.
Blue : DAPI
Dilution : 1:200

Anti-PPAR gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32803

GTX32803 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32803 PPAR gamma antibody.
Blue : DAPI
Dilution : 1:200

Anti-PPAR gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX32803

GTX32803 IHC-P Image

IHC-P analysis of rat heart tissue using GTX32803 PPAR gamma antibody.
Dilution : 1:100

Anti-PPAR gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32803

GTX32803 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32803 PPAR gamma antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Ezzeddini R et al. J Physiol Biochem 2021; 77 (2) : 249-260 Downregulation of fatty acid oxidation by involvement of HIF-1α and PPARγ in human gastric adenocarcinoma and related clinical significance.
Application : WB
Haiyan L et al. Brain Res Bull 2018; (Epub) Chronic * treatment reduces the anxiety-like behaviors induced by repeated restraint stress through modulating neuroinflammation.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399