APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:200 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human PPAR- gamma around the phosphorylation site of Ser112.
Purification
Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxisome proliferator activated receptor gamma , CIMT1 , GLM1 , NR1C3 , PPARG1 , PPARG2 , PPARgamma
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX02531 WB Image
WB analysis of extracts from mouse liver and rat lung tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
N peptide : Non-phospho peptide
P peptide : Phospho peptide
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GTX02531 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX02531 PPAR gamma (phospho Ser112) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400
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GTX02531 ICC/IF Image
ICC/IF analysis of PFA-fixed HepG2 cells (30min of 4μM Forskolin treatment) using GTX02531 PPAR gamma (phospho Ser112) antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
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GTX02531 IHC-P Image
IHC-P analysis of human colorectal cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX02531 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX02531 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX02531 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02531 PPAR gamma (phospho Ser112) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX02531 IHC-P Image
IHC-P analysis of human normal tissues adjacent to esophageal cancer using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX02531 IHC-P Image
IHC-P analysis of human colorectal cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX02531 IHC-P Image
IHC-P analysis of human brain tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX02531 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
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GTX02531 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
REFERENCE
There are currently no references for PPAR gamma (phospho Ser112) antibody (GTX02531). Be the first to share your publications with this product.
REVIEW
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