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PPAT antibody, N-term

Anti-PPAT antibody, N-term used in Western Blot (WB). GTX46492
Anti-PPAT antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX46492
Anti-PPAT antibody, N-term used in Western Blot (WB). GTX46492

Cat No. GTX46492

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Hamster
Package
50 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Hamster CHO K1 , HEK273 , HeLa , HepG2 , Human skin fibroblasts

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human PPAT

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoribosyl pyrophosphate amidotransferase , ATASE , GPAT , PRAT

Background

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-PPAT antibody, N-term used in Western Blot (WB). GTX46492

GTX46492 WB Image

WB analysis of HepG2 cells using GTX46492 PPAT antibody at 0.25μg/ml.
Lane A : marker
Lane B : HepG2 cells

Anti-PPAT antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX46492

GTX46492 IHC-P Image

IHC-P analysis of human kidney tissue using GTX46492 PPAT antibody at 4.0-8.0μg/ml.

Anti-PPAT antibody, N-term used in Western Blot (WB). GTX46492

GTX46492 WB Image

WB analysis of human skin fibroblasts, HepG2, HEK273 cells, HeLa cells, and hamster CHO K1 cells using GTX46492 PPAT antibody at 1:5000.
Lane :
1 :Human skin fibroblast (100μg)
2 : HepG2 (20μg)
3 : HEK293 (20μg)
4 : HeLa (20μg)
5 : Hamster CHO K1 (20μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379